Seroprevalence and molecular characterization of Hepatitis B Virus isolated from HIV patients attending referral center in Abuja Acc

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dc.contributor.authorObum-Nnadi, Charity
dc.contributor.authorJohn Chima Ndubisi
dc.contributor.authorChristy Chinyere Fredrick
dc.contributor.authorNathaniel Ejikeme Onyenwe
dc.contributor.authorNdidi Blessing Ohabughiro
dc.contributor.authorKelechukwu Sylvester Nwokorie
dc.contributor.authorObum Okechukwu Nnadi
dc.contributor.authorChristie Amechi Oyeka
dc.contributor.authorIkechukwu Okoli
dc.date.accessioned2025-01-10T22:51:02Z
dc.date.available2025-01-10T22:51:02Z
dc.date.issued2023-03-25
dc.description.abstractThe co-infection of the hepatitis B virus (HBV) with the human immunodeficiency virus (HIV) is one of the major challenges in the management of HIV. Both viral infections are among the clinical conditions of public health importance with high mortality and morbidity worldwide, especially in developing countries like Nigeria. They share common route of transmission which puts HIV positive individuals at risk of co-infection. It is therefore, necessary to document the molecular epidemiological dynamics of HBV among HIV patients in HIV referral center in Abuja, Nigeria. Using stratified random sampling, 200 HIV infected individuals on Antiretroviral Therapy ART were sampled in a referral center in Abuja, Nigeria and were evaluated for HBV using Rapid Test Device (RTD) strip for HBsAg, HBV Combo Rapid Test Cassette 5 Panel HBV. HBV and HIV viral DNA and RNA were extracted and characterized genotypically for HBV genotype A, B, C, D, E, and F specific genes by polymerase chain reaction (PCR) followed by sequencing. Obtained sequences were edited using the bioinformatics software. The overall prevalence of HBV among the study population was 10.0% (20/200), 15.0% (30/200), and 20.0% (40/200), for RTD strip, RTD HBV cassette and PCR, respectively. The genotypic characterization is HBV genotype E. Based on the study; the genotypic characterization is 100% of the study population. Gender, occupation, marital status, level of education and place of residence were the risk determinants of HBV among study population. The relatively high prevalence of HBV/HIV coinfection and the presence of circulating HBV genotype E has provided important epidemiological information on the molecular characteristics of HBV in HIV-infected in Nigeria, and this has important clinical relevance in the management of HBV/HIV co infection. Also, PCR has proven to be the most sensitive technique in the diagnosis of HBV. The findings of this research confirm that HBV is a major co-morbid infection and a threat to HIV patients. The PCR is the best method of diagnosis. The health sector, federal and state ministry of health should improve in providing PCR machines, creating awareness and vaccinating the populace against HBV to reduce the prevalence of these infections.
dc.description.sponsorshipSelf Sponsored
dc.identifier.citationCharity Ndidi Obum-Nnadi, Chika Maureen Ezenwa, John Chima Ndubisi, Christy Chinyere Fredrick, Nathaniel Ejikeme Onyenwe, Ndidi Blessing Ohabughiro, Kelechukwu Sylvester Nwokorie, Obum Okechukwu Nnadi, Christie Amechi Oyeka and Ikechukwu Okoli (2023).Seroprevalence and molecular characterization of Hepatitis B Virus isolated from HIV patients attending referral center in Abuja. International Research Journal of Public and Environmental Health. 10(1):17-26. DOI: https://doi.org/10.15739/irjpeh.23.002,https://www.journalissues.org/IRJPEH/
dc.identifier.issn2360-8803(e)
dc.identifier.issn2814-3817(p)
dc.identifier.urihttps://www.journalissues.org/IRJPEH/
dc.identifier.urihttps://repository.uniabuja.edu.ng/handle/123456789/650
dc.language.isoen
dc.publisherInternational Research Journal of Public and Environmental Health
dc.subjectHepatitis B virus
dc.subjectHuman Immunodeficiency Virus
dc.subjectCo-infection
dc.subjectreferral centre
dc.subjectprevalence
dc.titleSeroprevalence and molecular characterization of Hepatitis B Virus isolated from HIV patients attending referral center in Abuja Acc
dc.typeArticle
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