Production, Optimization and Partial Purification of Bacterial and Fungal Proteases for Animal Skin Dehairing: A Sustainable Development in Leather Production.

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dc.contributor.authorAransiola, Sesan Abiodun
dc.date.accessioned2025-01-13T20:56:59Z
dc.date.available2025-01-13T20:56:59Z
dc.date.issued2023
dc.description.abstractCrude microbial protease was produced from Bacillus subtilis KM5B and Aspergillus flavus KMAF1 after their 16S/18S rDNA molecular identification with 99.59 % and 98.99 % respectively through solid state fermentation in a medium containing hair, feathers and agro wastes. The crude protease after optimization had an optimum production with Bacillus subtilis of 35 mm, enzyme activity of 123.2 U/mL at 35 °C, pH 8.0 for 48 h while A. flavus recorded 18 mm and an enzyme activity of 122.1 U/ml at 30C, pH 7.0 for 120 h. The percentage protease recovery for the B. subtilis and A. flavus were 71.5 % and 94.8 %, fold purification at 1.5 and 2.0, total protein was 107.90 mg and 150.60 mg, enzyme activity was 125.06 U/ml and 122.01 U/ml, and specific activity was 1.159 U/mg and 0.81 U/mg respectively. Therefore, protease from the two organisms have potential to dehair animal skins in tanneries industries to improve the quality of leather without environmental pollution.
dc.identifier.citationUgbede, A.S., Abioye, O.P., Aransiola, S.A., Oyewole, O.A., Maddela, N.R. and Prasad, R., 2023. Production, optimization and partial purification of bacterial and fungal proteases for animal skin dehairing: A sustainable development in leather-making process. Bioresource Technology Reports, 24, p.101632.
dc.identifier.urihttps://doi.org/10.1016/j.biteb.2023.101632
dc.identifier.urihttps://repository.uniabuja.edu.ng/handle/123456789/669
dc.publisherBioresource Technology Reports
dc.titleProduction, Optimization and Partial Purification of Bacterial and Fungal Proteases for Animal Skin Dehairing: A Sustainable Development in Leather Production.
dc.typeArticle
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