Department of Microbiology - Scholarly Publications

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In Vitro Antimicrobial Activity and Phytochemical Screening of Jatropha curcas Seed Extract.
(International Research Journal of Pharmacy, 2011) Aransiola, S.A.
The antimicrobial effects of the methanol, ethyl acetate and hexane extracts of J.curcas seed at concentration ranging from 50-200mg/ml were tested against some pathogenic organisms using agar diffusion method; the extracts exhibited antimicrobial activities with the zones of inhibition ranging from 10-25, 8-23, 10-20 and 12-21(mg/ml) for Staphylococcus aureus, Escherichia coli, Salmonella typhi and Candida albican, respectively. The Minimum Inhibitory Concentration (MIC) which ranges from 3.13-12.5mg/ml was determined using the broth dilution method; the Minimum Bactericidal Concentration (MBC) ranges from 25-2.25mg/ml. The phytochemical analysis revealed the presence of alkaloid, glycosides,flavonoid and carbohydrate. The ability of the crude seed extracts of J.curcas to inhibit bacteria and fungi is an indication of its broad spectrum antimicrobial potential which may be employed in the management of microbial infections. It is necessary to determine the active dosage level so as to be able to formulate it into a pharmaceutical dosage for use in chemotherapy.
Production of laccase by Bacillus subtilis and Aspergillus niger for treatment of textile effluent
(Sustainable Chemistry for the Environment, 2025) Aransiola, S.A.
The improper disposal of textile effluents without effective treatment has adverse environmental, social, economic, and health impacts and as such, it is vital to find innovative technological solutions to reduce the negative consequences of textile effluents. Laccases are versatile multicopper enzymes found in plants, fungi and other microorganisms with wide applications especially in the textile and paper industry. This study examined the production of laccase from Bacillus subtilis and Aspergillus niger to remediate textile effluent. Both organisms were identified by molecular method and plate test method was used to evaluate laccase production by the two organisms. Rice bran emerged as the substrate of choice for laccase production. At optimum temperature (30°C), the highest laccase produced was 0.522 U/mL and 0.642 U/mL at 35°C for B. subtilis and A. niger respectively. The optimum pH level of 5 and 6 produced the highest laccase yield of 0.583 U/mL and 0.684 U/mL respectively. Significant improvements of laccases from B. subtilis and A. niger were observed on physicochemical analysis of TDS, pH, electrical conductivity, TSS, temperature and DO in treating textile effluent. Notably, these enzymes exhibited remarkable efficacy reduction in BOD (38 %), COD (14 %), and nitrate (23 %) levels in the effluent. The study underscores the efficacy of laccases from the microorganisms in treating textile effluent, with concentrations ranging from 10 to 30 U/mL proving effective. However, laccase produced from B. subtilis showed more remediation potential in textile effluent treatment compared to the one produced by A. niger
Roles of Marine Microbial Products to the Nigeria Economy.
(Springer Cham, 2024) Aransiola, S.A.
Metabolites and/or products originating from living things, including microbes, plants, and animals, are referred to as natural products. For thousands of years, people have used and consumed natural goods in various forms all across the world. Natural products derived from marine microbial sources have gained a lot of attention lately because of developments in X-ray crystallography, spectroscopy, deep-sea exploration technology, and other separation techniques. It is believed that the harsh conditions found in the sea, which include high or low temperatures, high pressure, low pH, and high salt concentrations, give marine microbial products their distinct physiological characteristics and unusual chemical structures. Because symbiotic microbes that produce natural products coexist with macroorganisms such as sponges and corals, it is also possible to extract marine natural products of microbial origins from them. Bacteria, fungi, viruses, microalgae, marine micro-animals, and symbiotic microorganisms are some of the major categories of marine microorganisms. More than 30,000 marine microbial products have been found since the first ones were isolated in the 1950s. Nigeria has a lot of potential for extracting lucrative marine microbial products because of its long coastline and big surface area. Products made from marine microorganisms are widely employed in the food and beverage, agricultural, cosmetic, and pharmaceutical industries. The Nigerian economy and its businesses stand to gain greatly from these broad use cases. However, to fully capitalize on these advantages, a thorough investigation of Nigeria’s maritime habitats should be carried out in conjunction with companies to find and market marine microbial goods.
Treatment of Pharmaceutical Effluent by Saccharomyces cerevisiae and Torulaspora delbrueckii Isolated from Spoilt Water Melon
(Research Journal of Environmental Toxicology, 2015) Aransiola, S.A.
A study was designed to assess the efficacy of yeast isolated from spoilt water melon in the biological treatment of pharmaceutical effluent. There were two yeast species identified as Saccharomyces cerevisiae and Torulaspora delbrueckii. Each of the yeast was inoculated into the effluent and incubated for 15 days. Saccharomyces cerevisiae shows the highest percentage reduction of 52.5%, 52.5% and 58.7% for BOD, COD and nitrate respectively of the pharmaceutical effluent and closely followed by the consortium which has 44.5%, 44.5% and 72.0% for BOD, COD and nitrate reduction, respectively. The least percentage reduction was displayed by Torulaspora delbrueckii with 38.3%, 38.3% and 79.7%. The study revealed that Saccharomyces cerevisiae isolated from spoilt water melon could be used in the biological treatment of pharmaceutical effluent.
Biofilms of Pathogenic Bacteria and Emerging Antibiofilm Strategies.
(Qhalikay. Journal of health sciences, 2022) Aransiola, S.A.
Biofilms act as physical barriers to the immune system and drugs used by the host, resulting in antimicrobial resistance. Biofilms reduce the chances of eradicating infections and can result in relapses and backsliding after conventional treatment. Biofilms have a big impact on food safety in the food industry; many foodborne outbreaks have been linked to pathogenic bacteria that can form a biofilm. Biofilm-associated infections can cause not only severe symptoms but also serious side effects and even death. The findings of an experimental study of pathogenic bacteria like Pseudomonas aeruginosa, Salmonella enteritidis, and Staphylococcus aureus forming biofilms are presented in this article. The process of biofilm formation and its development phases were displayed with preserved architectonics using light and scanning electron microscopes. The amount of biofilm formed was influenced by the growth medium as well as the incubation conditions and time. Biofilmforming microbes are a common cause of complicated and recurrent diseases, and they are usually linked to multidrug-resistant bacteria, which account for nearly 80% of all refractory nosocomial infections. Medical device- and tissue-associated biofilm infections are two types of biofilm infections. Understanding the pathogenesis and factors that contribute to biofilm formation, as well as the disruption and dispersal mechanisms of biofilms, will aid in the development of improved anti-biofilm strategies. Overall, this literature review can serve as a single source of information about microbial biofilm formation and mitigation strategies, which could be extremely useful to biofilm researchers.
Assessment of Water Contamination in Nigeria-Review
(Journal of Basic and Applied Research International, 2016) Aransiola, S.A.
Human exercises including industrialization and agricultural practices contributes significantly to the degradation and contamination of environment which contrarily affects the water bodies (streams and sea) that is a need forever. Universally, water contamination is a noteworthy issue far and wide. However aquatic resources comprises of greatly extensive variety of flora and fauna resources which offer an expansive exhibit of products with potential utilitarian application in farming and industries which renders profitable advantages and services. The slow poising of the waters is seen in Nigeria and the decimation of vegetation and agricultural land by industrial effluents release, agricultural release and oil spills. Regardless of general society and worldwide organizations' arrangement concentrate on this issue, the circumstance in Nigeria appears deteriorating and in this manner requests earnest and prompt considerations.
Bio-decolourization of Basic Fuchsin dye by Saccharomyces cerevisiae Isolated from Salt water and Palm wine
(Journal of Biology and Nature, 2015) Aransiola, S.A.
In this study, Saccharomyces cerevisiae isolated from palm wine and salt water was used to degrade 20 mg basic fuchsin dye for a period of 12 days under aerobic condition in 250 mL, 500 mL and 750 mL mineral salt media. The degree of decolorization of basic fuchsin was determined using UV-visible spectrophotometer with absorbance of 620 nm. At the end of twelve days, 60.39%, 41.29% and 24.47% (for Saccharomyces cerevisiae isolated from salt water) basic fuchsin decolorization by Saccharomyces cerevisiae were recorded and 72.61%, 48.88% and 33.92% (for Saccharomyces cerevisiae isolated from palm wine) basic fuchsin decolorization by the same organism were recorded in 250 mL, 500 mL and 750 mL concentrations at pH 6.5, respectively. The results suggest the potential of Saccharomyces cerevisiae for the treatment of waste water containing basic fuchsin.
Microbial Nanomaterial Synthesis: Types and Applications
(Springer Singapore, 2023) Aransiola, S.A.
The present chapter addresses synthesis of microbial nanomaterial, types, and applications. Nanomaterials can be made by combustion processes or can be purposely synthesized through scientific or engineering innovation to execute specific function. Production of nanomaterials through biogenic enzymatic processes has better quality compared to its counterpart produced via chemical processes. The biosynthesis of nanostructures involves a variety of biomolecules, including secondary metabolites, carbohydrates, and proteins released by different microbes. Extracellular polysaccharides aid the reduction of various metal ions and the stability of metal nanoparticles because proteins in bacterial membranes are crucial for titrating metal ions. Terpenoids and flavonoids, which are organic molecules, are efficient at stabilizing and sealing nanomaterials, which affect their overall composition, size, and form. Additionally, algae species and morphological diversity influence the secretion of nanostructures. Nanomaterials occupy a large surface area per volume ratio due to the arrangement of nanoscale size that contributed to their structures, together with indistinguishable proportions to biomolecules which enhance distinctive properties for numerous usages. Microbiologically produced nanomaterials offer a wide range of potential uses in a variety of industries, including agriculture, coatings, cosmetics, packaging, food, beverages, drug deliveries, bioremediation, biomedicine, diagnostics, and electronics production. Actually, scientists have started paying attention to this technology since the resulting nanoparticles displayed unique characteristics such as biocompatibility, a larger range of uses, cost-effective production techniques, and environmental sustainability. Additionally, a variety of natural biological resources, including plants, algae, fungi, actinomycetes, bacteria, viruses, and even secondary microbial metabolites, are utilized to manufacture nanoparticles.
Challenges and Future Prospects of Biotechnology
(Springer Nature Switzerland, 2021) Aransiola S.A
Biotechnology is the manipulation of living organisms or their components to produce useful products. It is an innovative, interdisciplinary field that impacts many sectors, including agriculture, veterinary, medicine, pharmaceutical, and fine chemicals production. It is one of the key enabling technologies for sustainable production. However, there are challenges and some promising future prospects on biotechnology. This chapter takes into account, various areas where biotechnology is challenging and where it could be a future solution if harness effectively.
Biosorption of Dyes in Wastewater Using Chitosan/cPolyethylene Nanoparticle as Adsorbent
(Discover Catalysis, 2024) Aransiola, S.A
Chitosan/low-density polyethylene (CHNP/LDPE) nanoparticles, sized at approximately 200 nm, were developed as efective adsorbents for removing dyes from wastewater. This study involved a systematic experimental investigation to evaluate the effects of several parameters: adsorbent dosage, contact time, temperature, pH, and initial dye concentration, all conducted in batch experiments at ambient temperature. The optimal adsorption conditions were identifed; specifcally, a pH of 5 was most efective for Methylene Blue (MB) dye, while a pH of 6 yielded the best results for Bromocresol Green (BG) dye. The highest removal efciency was observed with an initial dye concentration of 50 mg/L and an adsorbent dosage of 0.05 mg/L. Equilibrium studies indicated that the adsorption process conformed to the Langmuir isotherm model for single systems. Notably, MB exhibited a higher adsorption capacity of 147 mg/g compared to BG’s capacity of 142.8 mg/g. These fndings underscore the potential of CHNP/LDPE biocomposites as a novel biosorbent for dye removal in wastewater treatment applications, suggesting an efcient and environmentally friendly approach to managing dye pollutants.
Microbial enzymes for sustainable development - future guidelines
(Springer Nature Singapore, 2022) Aransiola S.A
Pollution of the environment is a significant threat to the health of humans and other living things. Traditional pollutant removal methods are ineffective at reducing pollution levels to acceptable levels. For pollutant remediation, biological methods are preferred due to their greater efficiency and biocompatibility. Bioremediation is the term for these low-cost, environmentally friendly methods of reducing pollution. Enzymes play the most important role in bioremediation methods. PAHs, azo dyes, polymers, organocyanides, lead, chromium, and mercury are among the organic and inorganic pollutants that enzymes can help to eliminate. Various enzymes from various species have been isolated. Recently, various enzymes isolated from various species have been used for pollutant bioremediation. Cytochrome P450s, laccases, hydrolases, dehalogenases, dehydrogenases, proteases, and lipases are some of the most common enzymes involved in bioremediation, and they have shown promise in the degradation of polymers, aromatic hydrocarbons, halogenated compounds, dyes, detergents, agrochemical compounds, and others. Mechanisms like oxidation, reduction, elimination, and ring-opening have aided recent advancements in the use of microbial enzymes for bioremediation.
Impact and Application of Microbial Biofilms in Water Sanitation
(CRC press, 2022) Aransiola S.A
Water availability for human consumption is largely declining, despite the fact that it is a basic requirement of life. Water resources are becoming increasingly scarce, and many are polluted by anthropogenic sources such as industrial effluents, agricultural waste, and household waste. As a result, before discharging wastewater into a natural water body, it must be treated to remove various contaminants found in the wastewater, such as floc, organic carbon, nutrients, inorganic salts, and heavy metals. This chapter briefly discusses the impact and significance of sanitation on public and environmental health; biofilm in wastewater treatment; the formation of polymeric matrix (biofilm) in pipelines in relation to physical, chemical, and biological processes; and the use of microbial biofilms in wastewater treatment and the agricultural sector, as well as methods for mediating biofilm growth by the choice of pipe material used and the disinfectant system employed.
Effects of cadmium and lead on the biodegradation of diesel-contaminated soil.
(Journal of Taibah University for Science, 2019) Aransiola, S.A
In this research, the soil samples were contaminated with 0.05% and 0.1% of cadmium and lead. Seventy pots containing 200 g of soil were polluted with 5% diesel (w/w) (10 g). The physicochemical parameters of the soil were determined. There was a decrease in the pH, and electrical conductivity but the nitrogen, phosphorous, organic carbon and organic matter increased during the 84 days of biodegradation. The bacteria isolated from diesel oil contaminated soil were species of Bacillus and Pseudomonas. The GC-MS analysis revealed a significant decrease in the alkane and an increase in the ester constituents of the biodegraded oil samples after 84 days. Diesel oil co-contaminated soil supplemented with 0.1% of cadmium and lead had a percentage degradation of 89.5% and 80.54% respectively while compared to the un-supplemented samples (28.36%) after 84 days. This indicates that lead and cadmium as co-contaminant in diesel contaminated soil improved the rate of biodegradation of diesel.
Biotoxin in Foods: Threats and Benefits
(CRC Press, 2025) Aransiola, S.A
Sources and techniques for biofuel generation.
(Elsevier, 2023) Aransiola, S.A
Environmental Management and Uses of Vinasse-Review
(Asian Journal of Current Research, 2016) Aransiola, S.A
Vinasse is the fluid waste material got from the fermentation procedure of alcohol refining, from crude materials, for example, maize, beet, molasses or juices of cassava, sugarcane, among others by the utilization of yeasts at a proportion of 12-15 liters for each liter of alcohol which has inorganic and organic loads. Vinasse is used in farming for cheap supplements sources, enhancing specialists and animal feed past the confinement of high chemical oxygen demand (COD) and biochemical oxygen demand (BOD) and aggregate dissolved salts content even after the toxin expulsion treatment. This substance has a low pH and high COD, which can bring about environmental desertification when unpredictably utilized as compost. Likewise, underground water pollution is being seen in a few districts.
Bio-Removal of Vat Dye from Textile Effluent by Candida tropicalis and Candida apis Isolated from Soil
(Expert Opin Environl Biol., 2015) Aransiola, S.A
The bio-removal of textile effluent (Vat dye) by yeasts isolated from soil was studied. A total of 5 yeasts were isolated and identified from soil (using morphological and biochemical characterization) to remove vat dye from textile effluent. These isolates were screened by inoculating them into 500 ml conical flask each containing sterile mineral salt media and 20 mg of vat dye. Of these isolates, Candida tropicalis and Candida apis stood out as potential dye decolorizes after incubation. Candida apis and Candida tropicalis removed 90.6% and 84.1% of color from the textile effluent after incubating for 25 days at 37º C. The textile effluent treated had initial high concentration of Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and Nitrate, above the standard acceptable limit which is an indication of high contamination. All the isolates used performed efficiently in reducing the concentration of these parameters in the textile effluent at neutral pH of 7.0. The Yeast isolates (Candida apis and Candida tropicalis) exhibited good potentials in the treatment of textile effluent. Candiada apis reduced the BOD from 1,425 mg/l to 272 mg/l COD from 3,550 mg/l to 679 mg/l while the Nitrate was reduced from 255 mg/l to 65 mg/l and Candida tropicalis reduced the BOD from 1,425 mg/l to 312 mg/L, the COD was reduced from 3,550 mg/l to 780 mg/l and the Nitrate was reduced from 255 mg/l to 78 mg/L. These microbial isolates can be recommended as good candidates for treatment of waste water, especially textile waste water.
Marine Bioprospecting for Sustainable Blue-bioeconomy,
(Springer Nature Cham Switzerland, 2024) Aransiola, S.A
This volume comprehensively discusses marine bioprospecting and its applications in the marine bioeconomy, specifically in clean energy generation, and in biomedical, industrial and agricultural sectors. The advent of modern technology, particularly advancements in deep-sea exploration and biotechnology, has enabled scientists to delve deeper into the ocean's depths and discover a treasure trove of unique organisms and compounds. This demonstrates that the rich history of human interactions with the oceans is firmly ingrained in marine bioprospecting. The blue-economy, which is a more accurate name for the systematic search for valuable substances and organisms in the water, has gained popularity in recent years as a possible route for sustainable economic development. One of the key driving factors behind marine bioprospecting is the growing realization that marine organisms possess unique biochemical compounds with the potential to revolutionize various industries. These compounds include novel enzymes, antimicrobial agents, bioactive molecules, and even potential pharmaceuticals. Readers will learn about the applications of these discoveries in bioremediation, wastewater treatment, and biofuel production, as well as the identification of natural substitutes for things that are detrimental to the environment, which include biodegradable plastics derived from marine microorganisms. The primary audience for the book will be governmental and international organizations, professionals, and economists, while the secondary audience will be professors and researchers in the fields of Chemistry, Biotechnology, Environmental Microbiology, and general Ocean Sciences.
Identification of methyl 18-methylnonadecanoate and bis(2-ethyl hexyl) phthalate as potential anticancer agents in Nauclea latifolia Smith through in silico molecular modeling and simulations
(Next Research, 2025) Aransiola, S.A
This study aims to explore the anticancer potential of compounds derived from Nauclea latifolia Smith using molecular docking and pharmacokinetic analysis. Gas chromatography-mass spectrometry (GC-MS) identified 25 bioactive compounds from the ethanol extract of N. latifolia leaves. Among these, methyl 18-methylnonadecanoate and bis(2-ethyl hexyl) phthalate were selected for further investigation due to their promising anticancer properties. The compounds were subjected to in silico molecular docking against two key cancer-related protein targets, ADAM17 and MAPK14, both implicated in cancer progression. The results revealed strong binding affinities for both compounds, suggesting potential inhibition of these targets. Pharmacokinetic profiling using Swiss ADMET indicated favorable drug-like properties, including high gastrointestinal absorption and non-carcinogenicity. These findings suggest that N. latifolia compounds, particularly methyl 18-methylnonadecanoate and bis(2-ethyl hexyl) phthalate, hold promises as candidates for anticancer therapy. Further in vitro and in vivo validation is required to confirm their efficacy and safety for therapeutic applications.
Production of Biosurfactants Using Pseudomonas aeruginosa for Biodegradation of Herbicide.
(The International Journal of Biotechnology, 2019) Aransiola, S.A
This study was aimed at isolating Pseudomonas aeruginosa from the soil to produce biosurfactant for degradation of herbicide. The soil sample was collected from oil polluted site in mechanic workshop, Minna, Niger State, Nigeria and transferred to the microbiology laboratory of the University in a sterile polyethylene bag. Pseudomonas aeruginosa was isolated from the soil and identified using microbiological and biochemical assays. The isolate was screened for biosurfactant production using haemolytic assay, drop collapse, oil spreading and emulsification test. Biosurfactant production was carried out using mineral salt medium for ten days. The ability of the biosurfactant produced by P. aeruginosa in degrading herbicide was tested on five concentrations of herbicide (5%, 10%, 30%, 50%, 70%) and the results showed biodegradation percentages as 56.4%, 53.1%, 32.6%, 29.8% and 26.2% respectively revealing that degradation was higher at lower concentrations. This suggests that herbicide could be biodegraded especially at low concentrations using biosurfactant produced by Pseudomonas aeruginosa.

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