Browsing by Author "Obum-Nnadi Charity Ndidi"

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    Analysis and Determination of the Proliferative Biomarker Potential of Ki-67 in Cervical Lession/Cervical Cancer using Immunohistochemical Method.
    (Journa of Coastal Life Medicine, 2023-11-02) Obum-Nnadi Charity Ndidi
    Background: Cervical cancer is believed to be one of the most widespread cancers worldwide with the utmost burden prevalent among women in areas where resources are scarce. Methods: The study population was from 10 years retrospective archival tissue blocks of cervical cancer among women in Abuja metropolis from 2005 to 2015 Histopathological diagnosis by H and E was made, from the 80 cases examined 20(25%) Cases were cervicitis (CC), 15(18.8%) were cervical intraepithelial neoplasia (CINI), 15(18.8%) were cervical intraepithelial neoplasia (CIN2) while 30(37.3%) cases were squamous cell carcinoma SCC They were examined immunohistochemically by Avidin Biotin Complex (ABC) method. Ki67 (proliferation marker). Results: A statistically significant rate of positivity was found in the expression of Ki-67 among all the categories of the studied cases. Conclusion: This study showed that most of the immunological markers examined can be used for identification of different stases involved in cancer progression, also for diagnosis of cancer, predictive prognosis and can be explore as therapy targets for cervicitis and Squamous cell carcinoma.
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    Phytochemical and Antibacterial Activities of Anacardium occidentale fruits extracts (Cashew) on two Drug Resistant Bacteria
    (International Journal of Science and Healthcare Research, 2020-06) Obum-Nnadi Charity Ndidi
    This present study was aimed at investigating the phytochemical and antibacterial activities of both aqueous and ethanolic extracts of Anacardium occidentale (cashew) fruit on Staphylococcus aureus and Escherichia coli. The Phytochemical constituents of this medicinal plant were carried out using standard methods. Agar well diffusion method was used to determine the antibacterial activity of the plant extracts. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the plant extracts on the test isolates were determined by the Broth dilution method. Phytochemical analysis showed that Phenols, alkaloids, anthraquinolones, flavonoids, glycosides, tannins, glycoside, terpenoids and tannins were present in both ethanolic and aqueous extracts of A. occidentale. The antibacterial activities of aqueous and ethanolic fruit extract of Anacardium occidentale showed that the mean zone diameter of inhibition for S. aureus on the different extracts was between the range of 11mm to 28mm while that of E. coli was between 17mm to 29mm. The MIC of different extracts of S. aureus was between 6.25mg/ml to 25mg/ml while that of E. coli was also between 6.25mg/ml to 25mg/ml. The MBC of different extracts of S. aureus isolates were between the ranges of 100 to 150mg/ml while that of E. coli was also between the range of 100 to 150mg/ml. The ethanol and aqueous extracts of Anacardium occidentale (Cashew) fruits are rich in wide range of secondary metabolites. Both extracts exhibited bactericidal activities against S. aureus and E. coli. The antibacterial efficacy of the fruit extracts of A. occidentale lends credence to ethno-medicinal use of the plant to treat various ailments.
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    Phytochemical and Antibacterial Activities of Ocimum Gratissimum on Some Selected Drug Resistant Bacteria
    (FUW Trends in Science & Technology Journal,, 2019-12) Obum-Nnadi Charity Ndidi
    The use of Ocimum gratissimum (scent leaf) as food and medicine could be attributed to its phytochemical and antimicrobial properties. This work is aimed at evaluating the phytochemicals and antibacterial activities of Ocimum gratissimum on some selected drug resistant bacteria. The antibacterial activities of the plant potent extracts were tested on the test isolates using Agar-well diffusion techniques. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts were determined according to the macro broth dilution technique. Phytochemical analysis of fresh and dried leaf extracts revealed the presence of tannins, alkaloids, flavonoids saponin, and terpeniods. Results obtained revealed that the cold water extracts of the fresh leaves was most potent, inhibiting all isolates with diameter zones of inhibition ranging from 16 to 20 mm, followed by ethanol extract of the dry leaves with zone range of 15 to 18 mm and hot water fresh leaf 11 to 18 mm, but E. coli showed total resistance to the cold water dry leaf extract. The extracts inhibited the growth of the bacterial isolates in a concentration dependent manner with MICs ranging between 50.5–150 mg/ml, while MBC ranged from 12.5–100 mg/ml. The findings from this study seem to provide the in vitro evidence that might justify O. gratissimum as a good candidate medicinal plant for further investigations, and that the active principles of the plant may be more polar in nature.