Browsing by Author "Fredrick, Christy Chinyere"

Now showing 1 - 3 of 3
  • Item
    A Comparative Study of the Different Diagnostics of Detecting Malaria and Typhoid Fever
    (Journal of Disease and Global Health, 2022-12-31) Fredrick, Christy Chinyere
    Aim: The study is aimed at comparing the different method of diagnosing plasmodium parasite and typhoid fever and also to know the most effective method of diagnosis. Methods: The different diagnostic method of analyzing malaria and typhoid were compared by using blood and stool samples of patients from Angwan Rogo community FCT, Abuja. The diagnostic method used for the analysis of malaria were Rapid diagnostic test kit (RDT) and microscopy diagnostic method, which are Field stain and Giemsa stain. The positivity rate of RDT was (12%), Field stain (88%) and Giemsa stain (94%). Typhoid fever was diagnosed using widal test and stool culture test. The results showed that age group 1-10 had the highest malaria prevalence of 70% followed by age group 21-30 with prevalence of 10%. The positive result for typhoid fever widal test is 63.3% while for stool culture is 90%. Conclusion: The result of the study shows that children are more prevalent to malaria. Therefore, laboratory personnels should not depend solely on widal test, but rather they should employ other diagnostic method such as stool culture which can differentiate salmonella infections from other infections for more diagnostic test result.
  • Item
    Assessment of Antibacterial Properties of Jatropha tanjorensis
    (Asian Journal of Applied Chemistry Research, 2022-12-29) Fredrick, Christy Chinyere
    Aim: This study aimed at evaluating the Phytochemical and antibacterial properties of Jatropha tanjorensis against selected test bacteria. Methods: The antibacterial properties were tested using the agar-well diffusion technique. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts were also determined. Phytochemical analysis of the hot and cold extracts revealed presence of flavonoids, glycosides, phenols, saponins, anthraquinones and tannis. Results: Results obtained revealed that the cold extracts were more effective than the hot extracts. Cold extracts of Jatropha tanjorensis leaf has antibacterial activity against E. coli and S. aureus. Hot extracts of Jatropha tanjorensis stem has very little antibacterial activity against S. marcescens at low concentrations with no MBC. Cold extract of Jatropha tanjorensis leaf was bactericidal for S. aureus at 250 mg/ml. Salmonella sp., E. coli, Pseudomonas sp., S. pyogenes, Serratia marcescens and Serratia marcescens were resistant to HE of Jatropha tanjorensis leaf and stem respectively. Conclusion: This study shows that there are phytochemicals present in Jatropha tanjorensis. The plant shows antibacterial properties against some bacteria, namely: Serratia marcescens, Staphylococcus aureus, Streptococcus pyogenes and Escherichia coli, indicating that they can be used as antimicrobials.
  • Item
    Bacteriological Evaluation of Semen Samples among Male Patients Attending a Tertiary Hospital in North-Central, Nigeria.
    (Advances in Bioresearch, 2023-05) Fredrick, Christy Chinyere
    Bacterial pathogen in semen is a productive health disorder emerging as a global serious medical and social issues that has much trauma, emotional instability and psychological stress of the affected individuals, this can be accessed by the quality and quantity of sperm cells as well as its structure. This research was carried out to detect bacteria pathogen in semen samples among male patients attending Bwari General Hospital Abuja. Semen samples from fifty (50) men were collected in sterile bottles for chemical and bacteriological analysis. A loopful of semen samples was inoculated immediately onto blood, chocolate and MacConkey agar immediately with the aid of a sterile wireloop for microbiological examination while the remainder was allowed to liquefy at 37oC for thirty minutes and was examined macroscopically for the following; appearance, volume, viscosity; and microscopically for the following; leucocytes, motility, sperm count and sperm morphology. The morphological sperm characteristics were studied by simple microscopy. Samples were cultured and bacteria isolates were molecularly characterized. Bacteria growth was positive for 37/50(74%) samples and negative for 13/50(26%) samples. The patients’demographical study was taken, statistically the age group between 41-50 years have the lowest prevalence rate of the infection. The younger age group and farmers have the highest rate of infection as compared to other occupation. Enterococcus faecalis, Lysinibacillus macrolides and Bacillus fusiformis with GenBank Accession number FJ378657.2, KX129780.1 and AY548954.1 respectively were molecularly identified. These organisms were previously mistaken for Staphylococcus aureus, Escherichia coli and Proteus species respectively. These bacteria showed a drastic effect on semen quality which could lead to infertility. Routine check up and personal hygiene is highly recommended as a control measure.