Aransiola, Sesan Abiodun2025-01-132025-01-132023https://doi.org/10.1016/j.sajb.2023.07.021https://repository.uniabuja.edu.ng/handle/123456789/673This study was aimed to examine the anticancer potential of Trigonella foenum-greacum seed methanol extract by analyzing its phytochemicals and its breast cancer cells effects. The anti-cancer efficacy of the extract was tested on fibroblast cells and MCF-7 through various cellular assays, including apoptosis, mitochondrial membrane potential, reactive oxygen species, cell cycle distribution, and cell cytotoxicity assay were all investigated. By using GC-MS analysis, fifty-five bioactive compounds were identified in the extract. When the cancer cell lines were treated with the extract, the typical apoptotic morphology was observed, this resulted in a dose-dependent reduction of cancer cell migration and adhesion. Flow cytometry studies revealed that the extract significantly induced apoptosis by triggering a change from G2/M to polyploidy (>G) at greater doses. Furthermore, the extract increased mitochondrial depolarization and ROS levels. This study demonstrates the extract's anti-cancer properties and highlights the need for further analysis to understand how Trigonella foenum-greacum seed extract induces program cell death through multiple signaling pathways at varying concentrations.enArticle