Fredrick, Christy Chinyere2025-01-102025-01-102023-05Ndidi, O.N.C., Chinyere, F.C. and ChidubemcKelechi, L., 2023. Bacteriological Evaluation of Semen Samples among Male Patients Attending a Tertiary Hospital in North-Central, Nigeria.Print ISSN 0976-4585;Online ISSN 2277-1573http://www.soeagra.com/abr.htmlhttps://repository.uniabuja.edu.ng/handle/123456789/654Bacterial pathogen in semen is a productive health disorder emerging as a global serious medical and social issues that has much trauma, emotional instability and psychological stress of the affected individuals, this can be accessed by the quality and quantity of sperm cells as well as its structure. This research was carried out to detect bacteria pathogen in semen samples among male patients attending Bwari General Hospital Abuja. Semen samples from fifty (50) men were collected in sterile bottles for chemical and bacteriological analysis. A loopful of semen samples was inoculated immediately onto blood, chocolate and MacConkey agar immediately with the aid of a sterile wireloop for microbiological examination while the remainder was allowed to liquefy at 37oC for thirty minutes and was examined macroscopically for the following; appearance, volume, viscosity; and microscopically for the following; leucocytes, motility, sperm count and sperm morphology. The morphological sperm characteristics were studied by simple microscopy. Samples were cultured and bacteria isolates were molecularly characterized. Bacteria growth was positive for 37/50(74%) samples and negative for 13/50(26%) samples. The patients’demographical study was taken, statistically the age group between 41-50 years have the lowest prevalence rate of the infection. The younger age group and farmers have the highest rate of infection as compared to other occupation. Enterococcus faecalis, Lysinibacillus macrolides and Bacillus fusiformis with GenBank Accession number FJ378657.2, KX129780.1 and AY548954.1 respectively were molecularly identified. These organisms were previously mistaken for Staphylococcus aureus, Escherichia coli and Proteus species respectively. These bacteria showed a drastic effect on semen quality which could lead to infertility. Routine check up and personal hygiene is highly recommended as a control measure.enBacterial pathogenSemenSperm countWBCsArticle